To purify and characterize Px1 and Px2 channels and hemichannels isolated from stably expressing mammalian cell lines and baculovirus expressed Sf9 cells, to address whether pannexin channels contain the same symmetry and oligomerization number as connexin channels, as has been hypothesized from electron micrographs of innexin structures. Connexin-based ap junctions are found in essentially all tissues of vertebrate body plans. Thus, there is great interest in understanding what role these newly discovered, putative gap junctions or hemichannels would play in complementing connexin-based intercellular channel communication in vertebrates.
It may well be that current textbook ideas on vertebrate gap junctions would need revising pending the outcome of these studies. Sokinsky's technical developments and achievements in correlative microscopies are already being disseminated in conferences and classrooms. This work will provide training opportunities for postdoctoral and undergraduate students. Some full text articles may not yet be available without a charge during the embargo administrative interval.
Some links on this page may take you to non-federal websites. Their policies may differ from this site.
Gaietta, J. Hu, R. Bruzzone, G. Dahl and G.
Boassa, D. Sosinsky, S. Ghassemzadeh, A.
Perez, Y. Jones, and M. Ambrosi, C. Boassa, J. Josef F. Arnulf Zweig. Greenfield Sluder. Stephen J Royle. Robert Hooke. Michael W Shaw. Julian Chen. Owen Meyer. John Stirling. Ancel Keys. Anand R. Stuart Feinstein. Lynne Cassimeris. Renata Basto. Arun K. Saurabh Jha. Paul Verkade. Joel Rothman. Thomas Mueller-Reichert. John J. William Detrich. Michael W. Terry D. Jennifer Waters. Arnaud Echard. Matthieu Piel.
Thomas Weimbs. Bestselling Series. Harry Potter.
Cellular Electron Microscopy: Volume 79
Popular Features. New Releases. Richard McIntosh. Free delivery worldwide. Description Recent advances in the imaging technique electron microscopy EM have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work.
Submit a Support Ticket
The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss.
Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.
Product details Format Hardback pages Dimensions x x Bestsellers in Microscopy. Add to basket.
NSF Award Search: Award# - Molecular and Cellular Structure of Gap Junctions
The Topography of Tears William H. In Search of Stardust Jon Larsen. Image quality improves with the square root of the number of areas averaged, and several thousand can be used.
Alternatively, a large number of images of identical, individual particles can be collected, the images oriented relative to one another, and their averages computed to accomplish the same task with particles that possess no symmetry. Image averaging based on symmetry or on galleries of distinct but identical particles or in the case of viruses and some other objects, on the use of both approaches together has made it possible to improve signal-to-noise ratios to the point that resolution has become as good or better than 1 nm e.
With this much image detail, it has been possible to integrate information from x-ray crystallography with images from cryo-EM and build up models for changes in the structure of complex molecular assemblies that accompany their biological function Agrawal et al. This important field of structural biology is rapidly expanding our knowledge of complex molecular mechanisms. The study of frozen-hydrated cells raises two issues that are not encountered during microscopy of isolated, subcellular assemblies: a cells are large and tremendously complex, so a huge amount of information is necessary to characterize them, and b no two cells, or even two large organelles like mitochondria, are sufficiently similar that their images can be simply averaged to enhance image signal-to-noise.
Thus, the methods for detailed characterization of cellular structure differ from those that are appropriate for macromolecular assemblies. To tackle the problem of cell size, one must either select tiny cells or find a way to collect appropriately small pieces of the cell of choice.
Related Cellular Electron Microscopy: 79 (Methods in Cell Biology)
Copyright 2019 - All Right Reserved